DR. BARUAH HEART CITY

                                                                                                   

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Unlimited poweR     of                  GenetiC EngineerinG

Inventor’s mouth.

Consequences of my research.

Introduction.

Mysteries in genetic sciences.

What is coronary artery disease?

Misconceptions in cardio-vascular sciences.

Foundation on which building of Baruah Applied Human.

Revolution in medical sciences rocked by Dr.D.R. Baruah,FRCSGlas.

Unfolding the mysteries in human genetic sciences.

Mysteries in human genetic sciences.

How does the mutation expresses in particular disease form?

Selection of patients for gene analysis

Eradication of heart disease & rarest of the rare diseases- Human genetic studies through sequencing of m-RNA.

Signal Transduction plays a major role during pre-bypass and post-bypass events.

How bypass surgery triggers signal transduction & phenotypically expressed.

Mutation

Selection of genes causing heart & other diseases.

Hypoxia, reactive oxygen species, intracellular calcium & Baruah syndrome.

Re-sequencing of the following genes to identify the mysteries.

First time on this planet– Genovac.

Baruah applied human genetic engineering- a choice of treatment for Cancer.

TGA-A New Method of Treatment of Complex Congenital Heart Disease.

Endocardial Cushion defect.

Genetic Engineering–To cure the rarest of the rare autoimmune.

First time on the Planet–Manifestation of Baruah Syndrome–Moyamoya

The rarest of the rare genetic disorder–Takayasu.

Isolated congenital Right Ventricular Hypertrophy.

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Applied Human Genetic Engineering - Vol.II

UNFOLDING THE MYSTERIES OF GENETIC SCIENCES IN INCURABLE DISEASES LIKE CORONARY ARTERY DISEASES, CANCER, & RAREST OF THE RARE DISEASES LIKE MOYAMOYA, SLE, TAKAYASU ETC.

CITY OF HUMAN GENOME A INSITUTE OF APPLIED HUMAN GENETIC ENGINEERING

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GeNeiTM

Name of Scientist :

 Dr.Dhani Ram Baruah

 Service :   

RT PCR, Cloning and Sequencing

 Service No :   

NC-08-003

 

                                       

 Figure 1: RT-PCR for amplification of gene Gene 1 (ERGIC)

                         1- SS-2,               5- C-1

               2- MS-3              6- S-11

                3-S-14                 7- S-16

 

                     

Figure 2: Confirmation of positive clones by restriction   digestion with EcoRI enzyme

                                 Clone 1 to 1

                                 M- 500 bp ladder

                                 Positive clone:  4,7,10,11,12,13,14 and 16

 

 

RAW SEQUENCE DATA for Sample C-1:

Clone No. 2:

Sequence with M13 Forward primer:

GGATGTATACGACTCACTATAGGGCGAATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGTTCTTTGCCACAAGATG

ACCCCATTGATGGCGCACACACCTGTCAGGAAGTGGGTGAAGGACCTGTGCTTCTCCGTCAGCTTCACCATCATGGGCGA

GAGCTCATAGAGGACGAAGACTCCGGGAAGGCCTTGGTCGCCCAACAGCCCATTGGCAACCTTCTCATGTCTGGTCACAG

AGAACTGATTTGTCCTCAGTACCTCTCCGTCCACCTTCATGTACACAGTGGGCACCACCTTCACAAAGTACTGGAACATC

ATGGAGGCTTGGGGCGCAGTGACATTGGTGTGGTCCAGGGGGTTCACAATGCCTGGATAGTCCTCCCCAAATGACAGGTG

CTGGATGTAGTGGGTCATGTTGATGTTGTCAAGGCCAAAGCTCTGCAAGTCATGGACGTGCACATGGGACTGCTGGAAGC

TCTTCCCAGGGGCAAAGTGGAAGTTTCCGGCCACCTTATTGACTTCCAAGAAGCCATACACCTGGCAGCCTTCATTCTTC

TGCTCCTGCATCTTCTGGCTGAAGCCCTCTCGCCGGCACTGCTCAATAGTATCTGGGTTCTTGAAGGCCCAGCCTCTACG

GCGATATGCCTCCCGCACATCTTCACAGGGTGTTACAGCACTTGATATCTTCTGCCTCACACCATAGCAGCCCTCACAGC

GATCAGGGTCCAGGGAGTCAGGGTCAAACACCGTCATCTCGACTTTCCCAAGCTCATGCCGCTCAGCCTCTGAGCTCAGG

GGATGCCATCTTTATCTAGTCGTTGCTTGAACAGGTTGTGTCCAATCCACTGCTGGTCCGGCTGCCATCATCATG
Sequence with M13 reverse primer:
 

CAGCTATGACCATGATTACGCCAGCTATTTAGGTGACACTATAGAATACTCAAGCTATGCATCCAACGCGTTGGGAGCTC

TCCCATATGGTCGACCTGCAGGCGGCCGCACTAGTGATATCCCGCGGCCATGGATGATGGCAGCCGGAACAGCAGTTGGA

TGTGGAACACAACCTGTTCAAGCAACGACTAGATAAAGATGGCATCCCCGTGAGCTCAGAGGCTGAGCGGCATGAGCTTG

GGAAAGTCGAGATGACGGTGTTTGACCCTGACTCCCTGGACCCTGATCGCTGTGAGGGCTGCTATGGTGCTGAGGCAGAA

GATATCAAGTGCTGTAACACCTGTGAAGATGTGCGGGAGGCATATCGCCGTAGAGGCTGGGCCTTCAAGAACCCAGATAC

TATTGAGCAGTGCCGGCGAGAGGGCTTCAGCCAGAAGATGCAGGAGCAGAAGAATGAAGGCTGCCAGGTGTATGGCTTCT

TGGAAGTCAATAAGGTGGCCGGAAACTTCCACTTTGCCCCTGGGAAGAGCTTCCAGCAGTCCCATGTGCACGTCCATGAC

TTGCAGAGCTTTGGCCTTGACAACATCAACATGACCCACTACATCCAGCACCTGTCATTTGGGGAGGACTATCCAGGCAT

TGTGAACCCCCTGGACCACACCAATGTCACTGCGCCCCAAGCCTCCATGATGTTCCAGTACTTTGTGAAGTGGTGCCCAC

TGTGTACATGAAGGGGACGAGAGGTACTGAGGACAATCAATCTCTGTGACAGACATGAAAGGTTGCCAATGGGCTGTTGG

GCGACCAGGCC

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