All patients were treated previously with Beta blockers, anti-diabetic agents either insulin or tablet form, lipid reducing drugs etc. All of them were on sorbitrate. Three patients were aged between 40-45years with left coronary artery blockages by 85-90% just immediately after origin. They all have inadequate ventricular performance. This is absolutely necessary to publish as the mystery in genetic sciences as till today none of the scientists and clinicians have found out the causes which are treated accordingly and publish which are to be correlated with clinical situation for permanent cure of CAD. The causes of CAD are published in thousands and thousands of research papers as change of life style and food habits. Excessive lipids are to be blamed for real pathology of occlusion of coronary arteries with compromising the forward blood flow leading to inadequate ventricular performance and premature death. None of the research papers have given any importance with peripheral arterial blockages which always compromising forward blood flow to the organs causing prematurely aging with increased gap between real and biological ages. Our research findings have suggested that peripheral arterial blockages are more important compared to coronary artery occlusion as it gives multi-organ failure and sufferings of the patients so badly that with bypass surgery of coronary arteries cannot improve the quality of life and premature death is made to be certain.

We have carried out genomic sequences of certain genes comparing with disease –free individuals where there is no mutations.

          Baruah biological molecules are isolated from edible medicinal plants, which were used by human for hundreds of years as vegetables or medicines with no side effects or complications. Those people who are using these plants found to have neither CAD nor cancer. They only suffer from infectious diseases. Our main purpose of the research is not only curing the dreaded disease, but to eradicate completely and to find out genetically at what age of the life it begins and how to combat. For eradicating purpose, we have selected 52 children with age between 13-16 years and few results of the entire group are presented here. We confirmed that these dreaded diseases begins at the age of 13-16years, which will be expressed after average between 15-20years, that is after the age of 28-36 years. From these experimentation, we confirmed that CAD can be cured and eradicate completely.

          A girl of 12 had an ependymoma of the brain, operated and treated with radiation therapy without success. Her maternal uncle, a man of 66years had a triple vessel bypass surgery six years prior to this incidence. His pre-bypass surgery symptoms recurred after 4 years and investigations were carried out and found that his all grafts were blocked with inadequate ventricular performance. Second bypass surgery was not possible and permanent pacemaker was implanted. Two years later, his ejection power of myocardium was further deteriorated in spite of having permanent pace maker and he had reached to the stage of end stage heart failure. His genomic sequences had revealed that he had an active mutation of gene responsible for CAD. Surprisingly, he had another gene having a silent mutation responsible for cancer. He did not suffer from cancer, but his niece is suffering from ependymoma.

          Homo sapiens mRNA endoplasmic reticulum golgi intercompartmental protein transcript variant two (ERGIC) Length 818 BP

This gene is intermediatery gene which is responsible for Cargo protein to enhance the transportation and distribution of the beneficial protein to the target cells to cure the disease and to eradicate further. Without activation of the gene, transportation of the Cargo protein cannot take place, therefore disease cannot be cured permanently or temporarily and question of eradication does not arise.

Baruah biological molecules are the key tools which activate these genes in form of mutation.

There was no mutation seen in this gene in healthy individuals, and it is not seen in diseased patients as well. But Baruah biological molecules allows the gene to be mutated for beneficial effect as described above. These mutations are temporary and cannot remain after three months after treating with Baruah biological molecules.It decays spontaneously as its function is no longer required. The mutations expressed as point mutations in two positions in 15 days and two months treated patients, most prominent is at 8th & 18th positions. At 8th position, adenine is replaced by Thymine where Aspartic acid is replaced by Valine . On the other hand, at 18th position, Cytosine is replaced by Thymine, which do not bring any change in amino acid expressing as silent mutation.

In conclusion, this particular gene is most important to cure each and every incurable and rarest of the rare diseases to cure permanently and its eradication.

             Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 1(lysophosphatidic acid acyltransferase, alpha) (AGPAT1), transcript variant 1, mRNA.

     None of the samples-healthy as well as diseased except those patients have undergone bypass surgeries showed mutations in this gene. Bypass surgery has acted as larger stimulus for signal transduction, which triggers these mutations and enhances the secondary blockages of coronary arteries and grafts. However, with Baruah biological molecules, these mutations are removed and blockages are cleared within 5-8 days with marked improvement in clinical conditions, with no recurrence and patients became normal. Patients became de-aged, reducing their biological age coinciding with real age. Ventricular performance is improved with adequate ejection. Therefore,it has been proven that bypass surgery is detrimental and mismatch surgical procedure.

     All the mutations in this gene is found in post-bypass surgery patients, where all the clones are showing point mutation at position 14, 142 and 194 nucleotide positions, where cytosine is replaced by thymine at 14th and 142 position and adenine is replaced by Guanine at 194th nucleotide position. We observed that 5th position amino acid Proline is replaced by Leucine, whereas, at 48th position, there is no change in amino acid Leucine, in spite of mutation in 142 nucleotide position and expressed as silent mutation. At 65th position, amino acid Aspergine is replaced by Serine. These mutations bring errors in metabolic pathways of lysophosphatidic acid synthesis and help in blocking the arterial system.

              Homo sapiens coatomer protein complex, subunit zeta 1 (COPZ1), mRNA.

In diseased condition, that is, in CAD, (patients with triple vessel disease waiting for bypass surgery) we have observed mutation in this gene.

It is known that Endoplasmic reticulum synthesizes lipids, protein for distribution, which goes to ERGIC for further modification. Study of this particular gene (ERGIC) does not show any major mutation, which I have already mentioned. Modified protein or fat from ERGIC is further transported to Golgi , where COPZ1- cargo protein gene is involved. But the present gene is showing conspicuous point mutations in CAD patients at 4 positions, namely, 50 and 176, where Adenine is replaced by cytosine & Guanine resp.(methionine replaced by Isoleucine & Isoleucine is replaced by Leucine resp.), 293 position where T is replaced by A, where Leucine is replaced by histidine and at 392 position, A is replaced by C where Aspartic Acid is replaced by Alanine. These all mutations are totally disappeared in 24 hours after Baruah applied human genetic engineering treatment using Baruah Biological molecules as tools.

It is important to note that, post-bypass surgery patients showed single point mutations at 6th position , where G is replaced by C and at amino acid level Methionine is replaced by Isoleucine. As I have already mentioned that bypass surgery is itself a large stimulus triggering a secondary occlusions,which is subsequently removed with Baruah genetic engineering.

m-RNA sequencing was done in volunteers aged between 13-16 years. We have selected these group of individuals, as this is the age for changing the physiology of human with hormonal imbalance and mutations were observed which are unstable and inconclusive, which could be detrimental or beneficial. However, this particular gene responsible for distribution of cargo protein from golgi is difficult to analyse at this age. It means this group of children, those who do not show any mutation will not suffer from CAD or those showing mutations will have blockages of coronary and peripheral arteries. Mutations are not observed in all the clones, which suggest that during this particular age, that is adolescent period, number of stimulus triggers signal transduction, bringing number of physiological changes and ultimately number of mutations. The number of factors such as duration of external stimuli, change of mental status, stress decides the stability of the mutation. If the mutations are detrimental, it will lead the child to suffer from dreaded disease in his/her adulthood or if mutation is beneficial, it will help the child to lead healthy normal life.

As I have already mentioned in adult genetic analysis, Baruah biological molecules have de-mutated number of detrimental mutations, which have helped to eradicate the dreaded diseases like CAD or cancer. That is why, I have modified Baruah biological molecules in vaccine form called Genovac- which is going to eradicate haywire mutations from adolescent age and prevent them from dreaded disease such as coronary artery disease or cancer in future.

              Homo sapiens G-protein signaling modulator 3 (AGS3-like, C. elegans) (GPSM3), mRNA.

Re-sequencing of this gene did not indicate any stable mutation, therefore, we conclude this gene is not involved in any of the diseased condition. Majority mutations are found in single clones, but no change in amino acids sequencing is found expressing as silent mutations.

               Homo sapiens Notch homolog 4 (Drosophila) (NOTCH4), mRNA.

This gene is under further genetic analysis.

Unfolding the Mysteries of genetic sciences- a conclusion

          Genetic science is based on two pillers- one is external stimulus and second is signal transduction. Survival of the building is entirely on the strength of the pillers, but the strength of the piller is determined by mixtures of various ingradient such as cement, sand and stone. Impurity of the ingradient makes the piller weaker and long-term survival of the building is questionable. Same way, I compare the impurities of the ingradients with qualities of the stimulus and its varieties. If the stimulus is the mistaken one, which will trigger mistaken signal transduction and ultimately signalling the molecules for mistaken mutations of m-RNA and ultimately it is expressed in diseased form of incurable variety. Similarly, if a stimulus is non-mistaken, it will trigger the correct signal transduction, which will be signalling non-mistaken form of sequencing in m-RNA, which will synthesize corrected form of protein, ultimately used for normal healthy mental and physical growth.

          Most astonishing mysteries in genetic sciences have not been found and not been described in the past and present. No one has described in the past, how coronary artery disease, which is now becoming an epidemic reaching as number one killer disease, and no one on this planet have discovered or invented the actual process of blockages genetically and its complete solution for permanent cure, as well as its eradication. There were number of hypothesis blaming the change of life style and food habits and trying to prove wrongly or rightly the lipid is fully responsible for blockages of coronary and peripheral arteries and lipids are of mainly dietary origin. We have found that these hypothesis are totally wrong and it is due to two genes. (gene 1 & 3). These genes are involved for any type of incurable diseases.

         Present medical science is ignorant about the root cause of the disease. What we have found is beyond the reach of todays medical sciences and beyond thinking of present clinician and scientist in this respect.Let me explain first why change of food habit and life style are not responsible for this disease?

         Mutations of these genes are mainly responsible for number of dreaded diseases. How these mutations occur?- It is the external stimulus in form of change of mental status and stress triggering the signal transduction, which may be mistaken or beneficial stimulus release the protein in form of enzyme or hormone binds with the receptors outside the cell on plasma membrane, which signals through the membrane to stimulate the similar situation in inner surface of the membrane, which gives signal to interact with various types of molecules for mutations. If the stimulus is mistaken one, mutation will be producing wrong protein synthesis and ultimately, it expresses in a diseased form.

         Change of mental status and stress causes the secretion of protein in form of hormone which changes the discoid shape to echinotic shape with loss of certain amount of surface area, which ultimately increases the amount of reactive oxygen species acting as a stimulus to trigger the signal transduction. This happens most commonly at the age between 13-16 years, several times in a day and innumerable times in a year. Ultimately mistaken protein is synthesized. When there is a bad signal and ultimately, the disease is expressed because of cargo protein cannot be transported to a final destination. At this point, coatomer protein is required to rescue this mutation by transporting or distributing to the golgi to particular site not only in coronary artery disease and peripheral arterial blockages, but also in other type of dreaded diseases. Therefore, de-ageing of the patient takes place at subcellular level and reduce the gap between real and biological age.

          Our genetic findings correlating with the clinical situation where blockages with a soft plaque disappears from peripheral and coronary arteries within 5-8 days, which has been proven by colour Doppler forward flow studies. Mutations are corrected within 24 hours. Patients feel much better clinically. Change of skin colour is the first to be noted and discomfort, pain, improvement of the liver function, improvement of the ventricular performance, patients look 20 years younger , their memory improved, regaining the sexual ability, which was disappeared. These clinical improvement after correction of the mutation observed during resequencing the genes after 24 hours of injection of Baruah biological molecules. The stability of the correction of the mutation have been followed up after resequencing of the genes with 15days, 2 months and 5 years of treatment. Results are given in isolated form. Therefore, I conclude that coronary artery disease is possible to cure completely with Baruah Applied human genetic engineering using Baruah molecules as tools and I have made complete eradication of this disease possible, which was impossible till today on this planet.

          As mutation of these gene (3), is solely responsible for the rarest of the rare diseases called Takayasu, Moyamoya, SLE.

          The causes of Takayasu was not known in the past when Mikito Takayasu,a Japanese opthalmologist had described in 1908 that one of his patient came to his clinic with sudden blindness. All investigations revealed the inflammation of the retinal arteries and it is due to vasculitis.

          However, it was not described at that time that it is developed from mistaken mutations of m-RNA. We confirmed that Takayasu is originated from mutation of m-RNA and it has a three types

  It involves arteries alone

  Involves venous system alone

  valvulitis- commonly mitral valve is involved.

          We found that it is a great mistake in medical science to describe that Takayasu had no treatment, as no one could find the causes and its treatment. Baruah biological molecules are used as tools to demutate the mistaken gene to correct our patients’ Takayasu disease both arterial, venous and valvular origin. We have treated 7 patients of these type, 5 females and two males. Two males were from same family, elder brother had suffered from Takayasu of arterial origin and younger brother, who was suffering from Takayasu of venous origin. Both of them were treated with Baruah biological molecules by correcting the mutations of the gene.

          Since inception of medical science, it has been known that rheumatic heart disease caused by streptococcus infection leads to mitral valve disease causing stenosis and incompetence. In third world countries, it is an epidemic of the people of poor economic conditions required mitral valve replacement. Baruah applied human genetic engineering using Baruah biological molecules surgical kind of intervention is not required for this disease. We have found that rheumatic heart disease is a mistaken terminology to be used. In reality, it is a another form of Takayasu, where mutation of the m-RNA is involved and Baruah biological molecules demutates the mutation and corrects this mitral valve pathology permanently.

          Initial streptococci infection causes the inflammation of mitral valve which act as stimulus for triggering the signal transduction causing the mutation of this particular gene and ultimately synthesizing a mistaken protein and this inflammatory mitral valve act as a continuous stimulus for signal transduction, thereby, mutation occurs and as a result mistaken protein is synthesized. The process of this takayasu valvulitis is similar to coronary and peripheral arterial blockages and once this mutation is corrected with Baruah biological molecules, the takayasu valvular disease becomes cured permanently by reducing fibrosis, removing the calcification as applicable and it allows the normal performance of the ventricle.

          SLE- systemic lupus erythromatosus is an autoimmune disease, where patients’ own antibodies cannot recognize its own DNA and there is a reaction between antigen and antibody form a new complex which circulates and destroys the organs at subcellular level. It was said that this disease cannot be cured, but with Baruah biological molecules, using as a tools correct the mutation and cures the disease permanently,

          Diabetes mellitus is one of the incurable disease becoming an epidemic. So far, it has no cure. It is classified as type I and Type II. In reality, there is no differentiation. The cause of diabetes are of genetic origin, it is the autoimmune response, which causes this disease.Baruah biological molecules cures this disease permanently.

          Moya moya is another rarest of the rare disease, is known to be incurable and its cause is not known. It is known to have no cause .according to past and present medical sciences. Our research in applied human genetic engineering suggested that this concept is totally wrong. It is due to germ line mutation where brain cells suffer from inadequate oxygenation due to occlusion of forward flow of the internal carotid arteries just at the site of entry into the cranium It is because of excessive collateral circulation which gives appearance of puff of smoke in imaging. (MRI). All signs and symptoms occur due to cerebral hypoxia and children are most commonly sufferer. We have experienced of treating the youngest patient of 3 years child. Surgical treatment is not successful. The mutation is corrected and transportation of cargo protein with assistance of gene 3 to target sites, which removes the blockages and allows the brain cells to have sufficient oxygenation. We have cured the several patients with identifying the causes and treating with Baruah biological molecules permanently. However, when the damage is permanent, it is only partially cleared. Those cases we have treated before reaching to permanent damage.