Fifty two human volunteers have been selected with age between 13-16 with consent of their parents. The parents wanted their children to be disease-free in future life time, particularly they were concerned about their heart diseases as it is one of the incurable disease and becoming epidemic in the world. All the volunteers were clinically found with no heart disease and it is substantiated by colour Doppler flow studies which is usual investigating procedure (Baruah investigating procedure to calculate CAD) to detect coronary and peripheral artery diseases. Their gene sequencing was carried out and recorded, particular reference to m-RNA isolation and sequencing.

         Twenty six volunteers were injected with Baruah biological molecules and 24 hours later, their m-RNA were isolated and their sequencing was carried out. One volunteer was 15, was found to be hyperactive and we suspected clinically that he will have mutated gene. Our clinical findings were confirmed and it was true. They were injected Baruah biological molecules once in every month with 4 doses and their m-RNA sequencing confirmed their demutations with change of amino acids.

          The whole procedure of extraction of m-RNA, & sequencing is done by Bangalore Genei, Bangalore, the project reports of five genes using 6 samples are presented here.

   Microarray experimentation with m-RNA isolation is carried out at Labindia, Gurgaon.

          We have found from our studies that signal transduction is responsible for entire scenario. Signal transduction is the process where external stimuli binds with the receptor located on the surface of cell membrane and sending the signals inside the cell to particular site in cytosol to interact with particular molecule to synthesize new protein for welfare of the cell or to cause mistaken metabolism expressing in a diseased form in later date. It requires at least 15-20years for full expression of the disease. In certain cases, these newly formed proteins do not change its structure during intermolecular reactions within cytoplasm and remains neutral and do not express in diseased form.

          Signal transduction is usually and always take place by external stimuli which is primarily a protein form. The hormonal overactive imbalance takes place during the period- age between 13-16 where change of mental status takes place with every individual. We found development of mental maturity of three months of old puppy is equivalent to 25 years old human.

          Even small external or extracellular stimuli can give a larger effect through signal transduction. Our m-RNA sequencing of all the volunteers suggest that the command of external stimuli triggers number of biochemical reactions which includes production of excess reactive oxygen species, which triggers the signal transduction in either way., which ultimately causing intermolecular reaction, leading to mutation in the process of translation and expressed in respective disease form during life once the conditions are favourable termed as active mutation.

          CAD is a generalised disease involving entire arterial tree, making the organs prematurely aged with increase of gap between biological & real age and certainly angioplasty, stenting and bypass surgery have become a mismatch surgical procedure, which increase the incidence of death. Presently, approx. 70million Indians are suffering from heart diseases and are on some form of surgical or medical treatment with no remedy. Premature death is inherent drawback of bypass surgery which can never be avoided, although it is proven that it cannot cure CAD, still it is an established wrong procedure in cardio-vascular practices.

          In case of cancer, it is a second major form of an epidemic and chemotherapy, radiation therapy & surgery are not been found to be suitable to treat this disease to give permanent cure. Our studies on Applied human genetic engineering has suggested that chemotherapy is a wrong medical treatment so is the radiation therapy. It destroys the venules & capillaries, leaving the normal cells to be deprived of oxygenation, which lead the patient to die earlier than the cancer usually kills them. We have been treating with our molecules responsible genes such as gene responsible for synthesizing cadherin protein, which stops further spread of cancer.

          Beta blocker is used to treat the essential hypertension with no permanent cure. If patient stops taking the beta blocker, they suffer from same hypertension, stroke, hypertensive nephropathy, cardiomyopathy etc.

          Type II diabetes is a misnomer terminology to be used and diabetes is always blamed for CAD. As I mentioned earlier, type II diabetes is a misnomer terminology to be used. Our studies have confirmed that it is the result of patient’s own autoimmune manifestation. We have found that diabetic II patients treated by other practitioners, are suffering from auto-immune diseases, which were not been detected or not been thought to investigate or ingnored. All cases of type II diabetes associated with autoimmune disease were treated by us with Baruah Applied human genetic engineering using Baruah biological molecules and cured. Surprisingly, the clinicians should have found out that organs like heart, kidney, liver are worst sufferers in Type II diabetes than Type I diabetes. Clinicians should have understood, which is very unfortunate that damage to these organs is not due to type II diabetes, but due to autoimmune disease and patients are always succumb to death prematurely after a long sufferings.

          Infact, heart disease comes first causing diabetes mellitus. B-cells are more sensitive to variations of oxygen than myocardial cells, which can withstand the deprivation of oxygen upto the occlusion of 30% of coronary arteries before it gives chest pain & discomfort. We have found functions of beta cells alters even at the level of 7% occlusion of pancreatic arteries where adequate synthesis of insulin becomes questionable. Therefore, our genomic studies have confirmed that it is the partial or complete occlusion of coronary and peripheral arteries those are responsible for diabetes mellitus and not other way around.

          It is thought that the gene therapy will help to redundant coronary artery bypass surgery, angioplasty, or stenting. The basic principal was to insert genes which are responsible for growth factor synthesis, which will carry out angiogenesis in the heart muscle. But it is a well known fact that coronary artery disease is not a localized disease. Entire arterial tree is involved with occlusion. The procedure of gene replacement/transfer therapy is a localized procedure, where "gene insertion" is done near the site of occlusion, but where entire arterial system is involved, is it possible to insert genes at each and every site? No. it was /is not possible. Gene insertion at every site will be a cumbersome procedure along with its procedural complications.