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 Unlimited poweR of GenetiC
EngineerinG
Inventor’s
mouth.
Consequences of my research.
Introduction.
Mysteries in
genetic sciences.
What is
coronary artery disease?
Misconceptions in cardio-vascular
sciences.
Foundation
on which building of Baruah Applied Human.
Revolution in medical sciences rocked by
Dr.D.R. Baruah,FRCSGlas.
Unfolding the
mysteries in human genetic sciences.
Mysteries in
human genetic sciences.
How does the
mutation expresses in particular disease form?
Selection of
patients for gene analysis
Eradication of
heart disease & rarest of the rare diseases- Human genetic studies
through sequencing of m-RNA.
Signal
Transduction plays a major role during pre-bypass and post-bypass
events.
How bypass
surgery triggers signal transduction & phenotypically expressed.
Mutation
Selection of
genes causing heart & other diseases.
Hypoxia,
reactive oxygen species, intracellular calcium & Baruah syndrome.
Re-sequencing
of the following genes to identify the mysteries.
First time on
this planet– Genovac.
Baruah applied
human genetic engineering- a choice of treatment for Cancer.
TGA-A New
Method of Treatment of Complex Congenital Heart Disease.
Endocardial Cushion defect.
Genetic
Engineering–To cure the rarest of the rare autoimmune.
First time on
the Planet–Manifestation of Baruah Syndrome–Moyamoya
The rarest of
the rare genetic disorder–Takayasu.
Isolated
congenital Right Ventricular Hypertrophy.
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Applied Human Genetic Engineering - Vol.II |
|
T he
rarest of the rare genetic disordeR
Takayasu & My new discoveries Mitral valve
disease is another form of takayasu - not of rheumatic
origin is cured permanently with Application of Baruah Applied Human Genetic engineering
Takayasu, the rarest of the rare
disorder is found in average of two cases in one million
of population.
 Introduction-
Dr. Mikito Takayasu was Japanese
ophthalmologist, who described this disease in 1908,
found a young man of 36 who came to him with sudden
blurred vision. During investigation, he found that his
retinal arteries were inflamed and blood supply to
retina was insufficient and subsequent investigation
revealed that the patient had pulseless wrist and feet.
The cause of disease was not known at that time.
Subsequently, there were many patients treated by other
doctors with anti-inflammatory drugs like cortisone, and
immunosuppressive drugs like Cyclophosphamide., but
success was not recorded.
What
is Vasculitis?
Our circulatory system is made of
network of blood vessels, including veins, arteries and
capillaries. If all of our blood vessels were joined end
to end, they would extend the length of nearly 60,000
miles. Arteries deliver oxygen-rich blood to our body’s
tissues, while veins return blood with increased amounts
of carbon dioxide — a waste product of metabolism — to
our heart and ultimately to lungs for purification.
In vasculitis, the blood vessels
become inflamed, which can cause the layers of the blood
vessel wall thickened. This narrows the lumen of blood
vessels, reducing the amount of forward blood flow— and
thereby reduces oxygen and vital nutrients — that
reaches to tissues. In some cases, a blood clot may form
in an affected blood vessel, obstructing blood flow.
Sometimes instead of becoming narrower, a blood vessel
may weaken and form a bulge (aneurysm).
What is Takayasu’s arteritis?- It is
a chronic inflammatory condition that affects the
largest blood vessel in the body (the aorta) and its
branches. Thus, the complications of Takayasu’s arise
directly or indirectly from damage to these blood
vessels. The vasculitides are classified according to
the size of blood vessel involved. Takayasu’s is one
type of classic "large vessel" vasculitis.
My contribution-
Dr. Takaysu’s description and
findings were strictly confined to inflammation of
arteries only. However, our research work has suggested
it is not true as it is a syndrome involving entire
circulatory system where veins are also involved leading
to reduction of inflow of blood to right heart and
ultimate left heart suffers leading to inadequate left
ventricular filling and causes death.
In heart valve disease like mitral
valve which is the commonest valve to be affected is due
to vasculitis or arteritis, although, it was thought
till now that this is the valvular disease caused by
streptococcus. We have found many cases of mitral and
aortic valve disease, where there is no history of
rheumatic valve disease, where streptococcal infection
to be blamed. This has been proven by curing these
valvular diseases with Baruah genetic engineering by
using Baruah biological molecules, therefore, we found
that mitral or aortic valve replacement is not always
the answer to cure valvular disease. The requirement of
replacement of heart valve is wrong diagnosis and
treatment.
We have treated 55 cases of mitral
incompetence and stenosis, 15 cases of aortic
incompetence and stenosis. 65% of mitral valve disease
had history of rheumatic fever and 100% aortic valve
disease had no history of rheumatic fever. These
patients were in long wait list of heart valve surgery.
They were all treated by Baruah genetic engineering
during the period of last 12 months.
Pathological findings and
overexpression of genes.
Histological findings reveals that
cell-mediated immunity plays an important role in the
pathogenetic
sequence leading
to vascular lesions. The cause of the disease is
unknown. Histological findings of
inflammatory cell
infiltration and necrosis of the arterial
vascular cells
strongly suggest that cell-mediated immunity
plays an
important role in the pathogenetic sequence leading to
the lesions.
In Takayasu arteritis, inflammatory
cell infiltration
tends to be
localized in the adventitia and outer part of
lymphocytes, natural killer (NK) cells, macrophages,
cytotoxic T
lymphocytes, and T helper cells. Natural killer cells,
may recognize
a 65-kD
heat-shock protein expressed in the aortic tissue and
induce vascular
cell injury by releasing the cytolytic factor
perforin.
Elevated plasma levels of interleukin
(IL)-6
and increased
expression of IL-6 and IL-1ß in
both circulating
mononuclear cells and tissue-infiltrating macrophages
have been
observed in patients with giant-cell arteritis.
IL-1ß and IL-6
are proinflammatory cytokines synthesized mainly
by activated
monocytes, macrophages, and T cells. These cytokines
have overlapping
activities such as B- and T-cell activation,
fibroblast
proliferation, and acute-phase protein synthesis. IL-1ß
induces a large number of cytokines and
upregulates the
expression of adhesion molecules on endothelial
cells, thus
promoting leukocyte interactions with the endothelium
; IL-6 enhances
T-cell cytotoxicity and natural killer cell activity.
Baruah biological molecules reduces
inflammatory activity by controlling interleukin
activity. physiological correction is achieved and
patient becomes asymptomatic.
In case of hypertrophy of the
ventricles either left or right, more systolic and
diastolic pressure is exerted because of the increased
rigidity of collagen protein and rigidity disappears.
Due to mutation of this particular gene, when metabolic
process is reversed, rigidity of the collagen protein
disappeared and behaviour of the ventricles become
physiological, so functionally, myocardium is working
normal, with achievement of disappearance of symptoms
and thereby, mental and physical growth of the child
becomes normal. These patients when they are usually
breathless and cyanosed on exertion and they do not do
well in the school.
How Baruah Genetic engineering work
in valvular disease ?
By injecting Baruah
biological molecules, the m-RNA are overexpressed which
engineers the diseased genes with following effects:-
1 It reduces the inflammation of
smallest capillaries and arteries and ultimately
disappears. Thereby, forward blood flow increases,
adequate oxygenation is given at subcellular level,
allows synthesize required amount of ATP, resulting
increase and improved strength of collagen bundles,
reduces fibrosis and ultimately valve becomes competent
with improved motions of the valve leaflet.
In valvular disease, myocardium is
usually stretched and it loses its ejection power. Once,
ATP is adequately synthesized and valves began to be
competent with disappearance of fibrosis and
calcification, ejection power of myocardium become
improved and increased and already dilated myocardium
reduces its size and bring it to normal and heart as a
whole functions physiological.
We have discovered in 5 cases not
having arteritis, but their entire venous system is
inflamed and inflow of venous blood was reduced in
certain parts and completely blocked in certain parts
particularly in legs. They have got swelling of legs and
arms with tachycardia , tiredness. Tachycardia was due
to inadequate ventricular filling.
Case no.1:-
33 year old man attended
our clinic with swelling of entire body with legs,
particularly both legs and arms. The swelling was not
pitting and it was almost solidified. We attempted
several times to take his venous blood from his both
arms and legs, but failed. We wanted to treat the
patient with genetic engineering, but his parents
refused to go ahead with the treatment and further
follow up was not possible.
Case 2-
Man of 26years suffered from
painful legs and swelling with breathlessness. He was
treated in CMCH, Vellore. But no diagnosis was made. His
treatment was symptomatic. One year later, he attended
our clinic and diagnosis was made as inflammation of
veins of legs and arms including subclavian and
innominate. He was suffering from tachycardia due to
insufficient ventricular filling. Colour Doppler flow
studies have suggested that his arterial flow was normal
and his entire venous system was inflamed and inflow of
blood flow to right heart was inadequate He had skin
discoloration (blackishness). He was treated with Baruah
biological molecules to engineer his m-RNA expressed
diseased genes and after 72 hours later, swelling of
legs and arm began to reduce and his tiredness and pain
on walking were reduced. Repeat colour Doppler flow
studies have suggested that his venous inflow was
adequate. His tachycardia was disappeared His skin
discolouration disappeared. There was no skin ulcer. He
was discharged. Post- discharged period was
uneventful.
Case 3 - A man of 68years, retired
government official, non-smoker and non-alcoholic had
suffered from multiple ulcerative skin lesions., not
responded to dermatological treatment.. He was having
swelling of legs, arms and entire body as a whole. He
was treated by dermatologist for one year with no
remedy. He attended our clinic six month ago with
complains of multiple itchy ulcers. His right cuff was
more painful than left during walking. Colour Doppler
flow studies have suggested that there was reduction of
forward arterial blood flow, more in right common
femoral along with venous inflow of both right and left
subclavian veins was reduced.
After genetic engineering with Baruah
biological molecules and his skin ulcers were healed and
he became a normal man.
Arteritis- Treated with Baruah
genetic engineering
A women of 33 years old had suffered
from pain in both legs, and dizziness. Her pulse in both
wrists was not detectable, her blodd pressure was not
recordable. Both anterial tibial arteries having absence
pulsation. She was investigated and diagnosed elsewhere.
Diagnosis was made on the basis of the findings of
peripheral angiography. Complete blockages were reported
in vertebral arteries and partial blockages in common
carotid arteries, which was making her dizzy. Both
subclavian arteries were blocked completely and so is
the femoral arteries. Muscles were functioning on
collateral circulation. Genetic engineering was
performed using Baruah bio logical
molecules. Her pulsation of radial and anterior tibial
arteries were palpable after 96 hours of the treatment.
Her chest pain disappeared and she remain, femoral and
dexcending aortans symptomless. (A
33 years old housewife
totally incapacitated with
Takayasu of arterial origin, suffered from severe
dizziness, pain and cramps in the legs. No pulsation in
popleteal and radial arteries, entire arterial system
was partially blocked. Now she is asymptomatic since
last 5 years and de-aged by 20 years.)
A 42 years old house wife had
suffered from severe pain during walking. Her tolerance
was upto 10 yards. She was breathlessness and tired with
chest pain.Colour Doppler flow studies revealed multiple
blockages in both subclavian, femoral, descending aorta.
After genetic engineering using Baruah biological
molecules, her all symptoms disappeared. Now she is
healthy leading normal life since last one year.
In conclusion, takayasu is not
strictly confined to to arteritis what was described by
Dr.Takayasu in the past. It affects veins and heart
valve as well. There was no treatment for takasu in the
past and now it is been treated successfully with Baruah
genetic engineering by use of Baruah biological
molecules as tools.
Pathological findings and
overexpression of genes.
Histological findings reveals that
cell-mediated immunity plays an important role in the pathogenetic
sequence leading
to vascular lesions. The cause of the disease is
unknown. Histological findings of
inflammatory cell
infiltration and necrosis of the arterial
vascular cells
strongly suggest that cell-mediated immunity
plays an
important role in the pathogenetic sequence leading to
the lesions.
In Takayasu arteritis, inflammatory
cell infiltration
tends to be
localized in the adventitia and outer part of
lymphocytes, natural killer (NK) cells, macrophages,
cytotoxic T
lymphocytes, and T helper cells. Natural killer cells,
may recognize
a 65-kD
heat-shock protein expressed in the aortic tissue and
induce vascular
cell injury by releasing the cytolytic factor
perforin.
Elevated plasma levels of interleukin
(IL)-6
and increased
expression of IL-6 and IL-1ß in
both circulating
mononuclear cells and tissue-infiltrating macrophages
have been
observed in patients with giant-cell arteritis.
IL-1ß and IL-6
are proinflammatory cytokines synthesized mainly
by activated
monocytes, macrophages, and T cells. These cytokines
have overlapping
activities such as B- and T-cell activation,
fibroblast
proliferation, and acute-phase protein synthesis. IL-1ß
induces a large number of cytokines and
upregulates the
expression of adhesion molecules on endothelial
cells, thus
promoting leukocyte interactions with the endothelium
; IL-6 enhances
T-cell cytotoxicity and natural killer cell activity.
Baruah biological molecules reduces
inflammatory activity by controlling interleukin
activity, thereby, physiological correction is achieved
and patient becomes asymptomatic.
In case of hypertrophy of the ventricles either left
or right, more systolic and diastolic pressure is
exerted because of the increased rigidity of collagen
protein and rigidity disappears. Due to mutation of this
particular gene, when metabolic process is reversed,
rigidity of the collagen protein disappeared and
behaviour of the ventricles become physiological, so
functionally, myocardium is working normal, with
achievement of disappearance of symptoms and thereby,
mental and physical growth of the child becomes normal.
These patients when they are usually breathless and
cyanosed on exertion and they do not do well in the
school.
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Copyright© DR Dhani Ram Baruah Heart City2007
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